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This is to determine the possibility of penetration of evaporated mercury to periodontal tissue after amalgam filling. Class 5 cavities on upper canine, total 40 teeth from 10 dogs, were prepared and amalgam alloy which contain about 10 Ci of radioactive mercury were inserted. The animals were sacrificed after the experiment. The teeth were routinely decalcified, sectioned, autoradiographed by means of emulsion and stained by H & E. 1. Blackened grains were found along collagen fiber space underneath gingival sulcus floor. 2. Mercury particle deposited more at cervical portion of periodontium than apical portion. 3. Blackened grains were found along the Sharpey’s fiber. Crest bone side was more evident than cement side. 4. Mercury penetration to periodontium of non-experimented tooth showed almost same findings as experimented one.
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